Albion's under-23s are targeting a place in the final.
Albion progressed to the last four of the competition following victories over Hailsham Town, Pagham and Crowborough Athletic, and will be aiming to secure their first success in this competition since 2013.
Brighton & Hove Albion’s under-23s are in Parafix Sussex Senior Cup semi-final action against Eastbourne Borough at Culver Road tonight, kick-off 7.15pm.
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Vic Bragg, Albion's professional development coach, said: "We know it won't be easy, but we're looking forward to it and are keen to do well.
“Eastbourne Borough are a good side with plenty of ex-pros but it's a chance to reach a cup final, so there's plenty to play for. At our level it's a bit of a balancing act in trying to teach the right things and also get results."
Ticket prices on the gate at Culver Road are priced at £10 for adults, £5 for concessions (over-65s and students with a valid ID card) and £3 for under-16s.
Follow live match updates from tonight’s game on Twitter (@OfficialBHAFC), and a full match report will be published after the match.
ALBION SQUAD: Collings (GK), Sanchez (GK), Collar, Cox, Cadman, A. Davies, Barker, Hall, Barclay, Hutchinson, Molumby, J. Davies, Tighe, Connolly, Mandroiu, Tilley.