Graham Potter’s side overcame Bristol Rovers in the previous round of the competition, while the Villans beat Crewe Alexandra 6-1 to set up this week’s trip to the Amex Stadium.
The sides will meet again in the Premier League next month at Villa Park, and there’s currently three points that separate the teams in England’s top flight, following the opening six matches of the season.
Albion shared the spoils in a goalless draw against Newcastle at St James’ Park on Saturday, while Aston Villa, who took the lead twice against ten-man Arsenal at the Emirates Stadium, conceded two late goals to lose 3-2 in north London.
If the match ends all square after 90 minutes, there will be no extra time and the game will be decided by a penalty shootout, using the traditional ABAB format.
VAR will not be in use at the Amex, and will only be in operation for the semi-finals and final of this year's competition.
Potter confirmed that both Neal Maupay [knock] and Glenn Murray [ankle] are unlikely to feature on Wednesday night, as both players work towards Saturday's match against Chelsea at Stamford Bridge.
Leon Balogun, Jose Izquierdo, Alireza Jahanbakhsh and Leandro Trossard are all unavailable, while Bernardo edges towards making a return.
For the Villans, Jonathan Kodjia and James Chester are both expected to miss out through injury.
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Second round action…
Glenn Murray popped up in the second minute of second-half stoppage time to book Albion’s place in the third round of the Carabao Cup, as he stroked home the winning goal at the Memorial Stadium.
Aaron Connolly, on his full senior debut, put the Seagulls ahead in the 55th minute, before Tom Nichols equalised for the League One side nine minutes later.
But it was Murray who showed his experience in the closing stages to meet a Steven Alzate cross and finish with ease to cancel out the prospect of a decisive penalty shootout.
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Our last fixture…
Albion line-up: Ryan; Dunk, Webster, Burn; Alzate, Stephens, Propper, Montoya; Mooy (Connolly 70), Gross (Bong 82), Maupay (Bissouma 82).
Subs not used: Button (GK), Duffy, Baluta, Murray.
Their last fixture…
Pepe 59 pen
Aston Villa 2
Villa line-up: Heaton; Gilbert, Engels, Mings, Taylor; McGinn, Nakamba (Hourihane 85), Grealish; Trezeguet (Elmohamady 68), Wesley, El Ghazi (Lansbury 86).
Subs not used: Steer (GK), Konsa, Jota, Davis.
Villa’s player to watch…
The West Midlanders boosted their attacking ranks over the summer with the acquisition of Club Brugge striker Wesley, who arrived at Villa Park for a club-record initial fee of £22m.
The 22-year-old fired his first goal for the club in last month’s 2-0 victory over Everton, before also finding the back of the net in Sunday’s narrow defeat to Arsenal at the Emirates Stadium.
Wesley helped Club Brugge to a second-placed finish in the 2018/19 season — scoring ten league goals and a further three across the Champions League and Europa League.
The Brazilian has shown early signs of his quality, but will no doubt be desperate to add to his steady goal tally if selected for action in the Carabao Cup on Wednesday.
The match is currently on general sale, however to avoid a price increase of £5, supporters should purchase their tickets before Wednesday.
Matchday Price: (from 9am on Wednesday 25th September)
Click here to purchase tickets for the game.
*Booking fees of £1.50 per ticket apply by phone and in person, £1 per ticket booking fee applies online for tickets that are printed and posted/held for collection. Tickets purchased online using print-at-home are exempt from booking fees.