Premier League action takes a backseat until Saturday’s visit to Manchester City, and Graham Potter will look to make full use of his playing squad, ahead of the international break.
Defeat to Southampton on Saturday afternoon ended our unbeaten start to the campaign, while the Pirates head into the match on the back of consecutive league victories over Tranmere Rovers and Oxford United.
Rovers reached the second round after beating Cheltenham Town 3-0 earlier this month, while Albion join 12 other Premier League clubs entering the competition at this stage.
If the match ends all square after 90 minutes, there will be no extra time and the game will be decided by a penalty shootout, using the traditional ABAB format.
VAR will not be in use at the Memorial Stadium, and will only be in operation for the semi-finals and final of this year's competition.
Potter confirmed there will be opportunities for those who haven't played as regularly this season, including the likes of summer signing Adam Webster.
Yves Bissouma, Jose Izquierdo and Ezequiel Schelotto all remain sidelined for Albion through injury.
Last time we met…
Bristol Rovers 2
Hoskins 2, 86
Bolger 73 og
Despite two goals from Will Hoskins, who switched to the Amex Stadium a few months later, Albion hit Rovers with four goals at the Memorial Ground to strengthen their challenge for the League One title.
Ashley Barnes found the back of the net to level the game, before Elliott Bennett stroked home to put Albion ahead. Cian Bolger’s own goal strengthened the lead and Glenn Murray rounded off the scoring for Albion.
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Our last fixture…
Albion line-up: Ryan; Duffy, Dunk, Burn; Montoya, Stephens (Gross 78), Propper, March (Locadia 68); Maupay (Murray 75), Andone, Trossard.
Subs not used: Button (GK), Bernardo, Webster, Mooy.
Their last fixture…
Bristol Rovers 3
Oxford United 1
Bristol Rovers line-up: Jaakkola; Hare, Davies, Craig, Kilgour, Leahy; Clarke, Upson, Ogogo; Clarke-Harris, Smith.
Subs not used: Stappershoef (GK), Little, Bennett, Menayese.
Their own to watch…
After signing for Bristol Rovers from Coventry City at the start of the year, Jordan Clarke-Harris scored 11 goals in 16 games to end the 2018/19 campaign.
The former Rotherham United forward, who had Championship experience with the Millers, has scored twice for the Pirates so far this season, including a goal against Oxford United over the weekend.
Alongside Tyler Smith, who bagged a brace in the last round of the Carabao Cup, Clarke-Harris is proving a real threat for Graham Coughlan’s side.